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One week prior to mating, mice were placed on either a control AIN76A diet (#110098 Dyets Inc., Bethlehem, PA) consisting of carbohydrates (66.00%), protein (20.30%), and fat (5.00%), [as sucrose (500g/kg), casein (200g/kg), cornstarch (150g/kg), cellulose (50g/kg), corn oil (50g/kg), mineral mix #200000 (35g/kg), vitamin mix #300050 (10g/kg), DL-methionine (3g/kg), and choline chloride (1.1g/kg)], or on a choline-supplemented AIN76A diet (#110184 Dyets Inc., Bethlehem, PA), differing only by containing 5.0 g/kg of choline chloride, creating control diet and choline-supplemented groups. Read more…

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